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. 2014 Mar 31;65(9):2449–2461. doi: 10.1093/jxb/eru139

Fig. 4.

Fig. 4.

Ectopic expression of CYCD3;2 or CYA2;3 induces GC subdivisions. (A) The loss of FAMA function phenotype is epistatic to FAMA:CYCD3;2, presumably because fama-1 blocks the acquisition of GC fate. Inset: a fama-1 tumour. (B) FAMA:CYCD3;2 and flp-1 myb88 together show additive phenotypes of stomatal clusters and GC subdivisions. Inset: flp-1 myb88 stomatal cluster. (C) CDKB1;1:GUS, a marker for the stomatal terminal differentiation stage, expressed from the transition stage of GMC to GC in the wild type. Note the weak GUS expression in a mature stoma (an arrow). (D) TMM:TMM-GFP expression in stomatal lineage cells in the wild type. (E) E1728, a mature GC marker expressed in wild-type GCs. (F) Equally enhanced CDKB1;1:GUS expression in a subdivided FAMA:CYCD3;2 stoma. Arrowheads point to the extra cell division planes. (G) No TMM:TMM-GFP expression in FAMA:CYCD3;2-subdivided GCs. (H) Subdivided GCs induced by FAMA:CYCD3;2 eventually exhibit a GC fate as shown by the expression of the E1728 marker. An arrow points to the subdivision plane. (I) Expression of CDKB1;1:GUS in a stoma with four GCs of FAMA:CYCA2;3. An arrow points to the GC showing a differential higher GUS staining than its sister cell. (J) Ectopic expression of TMM:TMM-GFP could be found in one of the subdivided GCs, indicated by an arrow. (K) FAMA:CYCA2;3-induced GC subdivision mimics FAMA:CYCD3;2, but some of them were unequal divisions. E1728 GFP fluorescence is from a mature GC but was absent in another GC (arrow). Bars=20 μm. (This figure is available in colour at JXB online.)