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. 2014 Apr 10;65(9):2521–2531. doi: 10.1093/jxb/eru140

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Expression of BRP4. (A) RT-PCR analysis of BRP4 expression in various organs. Rt, root; St, stem; Yl, young leaf; Ol, old leaf; In, inflorescence; Fb, flower bud; Ysi, young silique. (B) Expression of BRP4 in anthers at the anther different developmental stages assayed by qRT-PCR. The data are from three biological replicates and are presented as means±SD. (C–J) BRP4 mRNA accumulation assayed by RNA in situ hybridization. RNA in situ hybridization was performed with anthers at stage 5 (C), stage 6 (D), stage 7 (E), stage 8 (F), stage 9 (G), stage 11 (H), and stage 12 (I). The anther at stage 7 hybridized with a BRP4 sense probe is shown as an example negative control (J). Bars, 20 µm.