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. 2014 Apr 10;65(9):2521–2531. doi: 10.1093/jxb/eru140

Fig. 3.

Fig. 3.

Knockdown of BRP4 expression causes an aborted pollen phenotype. (A, B) Morphology of mature pollen from WT (A) and transgenic proBRP4:BRP4 RNAi L7 plants (B). (C, D) Alexander staining of pollen from WT (C) and L7 (D) plants. Bar, 20 µm. (E) Percentage of the shrunken pollen in WT and four independent T3 transgenic proBRP4:BRP4 RNAi lines (L3, L7, L9, and L10). (F) qRT-PCR analysis of the expression of BRP4 in WT and proBRP4:BRP4 RNAi lines as described in (E). The data are from three biological replicates and are presented as means±SD (Student’s t-test, **P<0.01).