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. 2014 Apr 10;65(9):2521–2531. doi: 10.1093/jxb/eru140

Fig. 5.

Fig. 5.

ORC6 acts downstream of BRP4 during male gametogenesis. (A) qRT-PCR analysis of cell division-related genes in WT and transgenic proBRP4:BRP4 RNAi inflorescences. The data are from three biological replicates and are presented as means±SD (Student’s t-test, **P<0.01). Note the decreased expression of ORC6 in the L3 and L7 plants. (B) Alexander staining of mature pollen (MP) from WT and transgenic proBRP4:ORC6 RNAi plants. Bars, 20 µm. (C) DAPI staining of pollen at the bicellular and tricellular stages from transgenic proBRP4:ORC6 RNAi anthers. The red arrow shows abnormal uninucleate pollen, the green arrow shows bicellular-like pollen, and the yellow arrow shows shrunken pollen with no DAPI staining. Bars, 20 µm. (D) Percentage of abnormal pollen in transgenic proBRP4:ORC6 RNAi L4 and L5 anthers. BP, bicellular pollen; TP, tricellular pollen. At the BP stage, about 1500 pollen grains were examined, and approximately 2500 pollen grains were examined at the TP stage. (E) DNA content analysis of WT generative-cell nuclei at prophase (n=42) and undivided generative-like cell nuclei at anthesis in proBRP4:ORC6 RNAi L4 (n=57) and L5 (n=53).