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. 2014 May 5;6(5):2012–2027. doi: 10.3390/v6052012

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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IAPV-virion proteins are synthesized in larvae injected with IAPV extracts of bees from CCD-colonies. Immunoblot analysis of protein extracted from virus-free larvae injected with IAPV extracts from colony samples (indicated above the figure). Panels (a) and (b), injection of IAPV samples from July 2011 and February 2012, respectively. All viral samples were separated by PAGE and immunoblotted with anti-VP3 antiserum. Arrows, virion proteins recognized by the serum. Molecular size markers are indicated on the right. mi, control mock-injected larvae with extracts derived from healthy bees.