Table 1. Summary of Mutations and Coverage Obtained for the EMS-Mutagenized Individuals in Each Capture Reaction.

Capture Name	2	3	4	5	Wheat
EMS	20	10	14	28	6
EMS seed contaminant	0	0	0	2	0
No read obtained	0	0	0	2	0
Control	0	0	0	1	1
Genotype	0	0	8	0	0
Total	20	10	22	32	7
Percentage of base pairs on target	64.5 ± 2.3	72.6 ± 0.6	57.3 ± 0.4	24.1 ± 0.6	49.1 ± 1.2
Mean no. of reads (million)	12.1 ± 7.8	16.2 ± 3.0	3.9 ± 1.3	11.2 ± 3.1	33.0 ± 5.2
Sequencing lanesa	1	1	0.5	1	1
Fold increase coverage on versus off targeted regions	15.8 ± 1.6	22.9 ± 0.7	11.6 ± 0.2	2.7 ± 0.1	n/a
Mean no. of mutations/individual	246 ± 176	316 ± 378	70 ± 59	508 ± 361	1178 ± 187b
Mean no. of het. mut./Mb	3.9 ± 2.9	4.6 ± 5.1	5.0 ± 4.1	5.8 ± 3.5	16.9
Mean no. of hom. mut./Mb	2.0 ± 0.8	2.5 ± 2.8	1.7 ± 0.9	3.1 ± 1.8	3.2
Overall mutation rate (no. of mut./Mb)	5.9 ± 3.4	7.0 ± 7.8	6.7 ± 4.5	8.9 ± 5.2	20.1
Percentage of deleterious mutations	18.4 ± 2.5	19.4 ± 3.6	21.3 ± 6.2	11.7 ± 3.0	n/a

All statistics are calculated per sample and averaged per capture reaction. Mean standard deviations are indicated. Control nonmutagenized samples, genotypes, and individuals suspected to be seed contaminants are not included in the statistics (see Methods). n/a, not available.

^a100 PE HiSeq 2000 sequencing lane.

^bThe average number of mutations per wheat lines was calculated at a minimum mutant allele coverage of 4 for heterozygous mutations and a minimum mutant allele coverage of 7 for heterozygous mutations (adjusted for 3% false positive rate, based on the Kronos control sample).