qRT-PCR Analyses of SCRb, SRKb, and ARC1 Expression in A. thaliana
Col-0 and Sha Transgenic Lines.
(A) Relative levels of SCRb expression in mature anthers from the different genotypes.
(B) Relative levels of SRKb expression in the upper half of mature pistils from the different genotypes.
(C) Relative levels of ARC1expression in the SCRb-SRKb + Al-ARC1 (light gray) or SCRb-SRKb + Bn-ARC1 (dark gray) lines.
The relative levels of SCRb, SRKb, and ARC1 expression were normalized to the expression of two control genes, Elf1α and TUB4. An A. lyrata stigma RNA sample was included as a positive control for the relative levels of wild-type Al-SRK1 and Al-ARC1. Means from six technical replicates are shown. Error bars indicate se. SC, self-compatible; SI, self-incompatible.