Table 1. Self-Incompatibility Phenotypes of Transgenic A. thaliana in the Col-0 Ecotype.

Transgenes	Self-Incompatible		Self-Compatible	Total No. T0 Plants Tested
	Strong	Moderate
SCRb-SRKb	0	0	20	20
SCRb-SRKb + Al-ARC1	5	11	4	20
SCRb-SRKb + Bn-ARC1	7	6	7	20

Phenotypes were assessed by pollinating emasculated late stage 13 pistils (open flowers) with self-pollen for 2 h, followed by aniline blue staining of the pollinated pistils to view pollen tube growth. The T0 plants were scored as “strong self-incompatible” if <10 pollen tubes/pistil were observed or “moderate self-incompatible” if >10 pollen tubes/pistil were observed and there was a clear reduction in the number of pollen tubes relative to the self-compatible controls (n = 3 pistils/T0 plant).