Table 3. H2A.Z Deposition at DMC1 Requires ARP6.
Gene |
ChIP-qPCR Measurement of H2A.Z Enrichment |
Fold Difference in H2A.Z Enrichment (WT/arp6-1) | P Value | |||
---|---|---|---|---|---|---|
WT |
arp6-1 |
|||||
LSM | sd | LSM | sd | |||
DMC1 | ||||||
1 | 1.89e | 0.59 | 1.76ab | 0.88 | 1.07 | 0.377 |
2 | 1.19f | 0.25 | 1.02d | 0.13 | 1.17 | 0.033 |
3 | 1.31f | 0.44 | 1.56bc | 0.41 | 0.84 | 0.036 |
4 | 1.14f | 0.44 | 0.80def | 0.42 | 1.43 | 0.041 |
5 | 1.42f | 0.59 | 1.39c | 0.62 | 1.02 | 0.898 |
6 | 0.78g | 0.30 | 0.72ef | 0.41 | 1.08 | 0.550 |
7 | 2.48d | 0.86 | 0.99d | 0.56 | 2.51 | <0.001 |
8 | 3.41b | 1.41 | 0.97de | 0.21 | 3.52 | <0.001 |
9 | 3.97a | 1.88 | 1.88a | 1.36 | 2.11 | <0.001 |
FLC | ||||||
A | 3.00c | 2.04 | 0.64f | 0.67 | 4.69 | <0.001 |
Numbers in the first column refer to DMC1 genomic region assayed by quantitative PCR for H2A.Z enrichment (as shown in Supplemental Figures 8 and 9). “A” is the genomic region immediately downstream of the transcription start site assayed by quantitative PCR for H2A.Z enrichment in FLC. Least-squares mean (LSM) of H2A.Z enrichment in eight technical replicates (four biological replicates). Within a genotype, any pair of least-squares means that do not share the same letter are significantly different (P ≤ 0.05) from each other in pairwise comparisons for H2A.Z enrichment. The final column is the probability value associated with a pairwise test between LSMs of H2A.Z enrichment at each locus between wild-type (WT) and arp6-1 floral buds. P values ≤ 0.05 indicate significant differences between genotypes.