Figure 1.

Cuticular Wax Accumulation in Arabidopsis under Dark Conditions.

(A) Cuticular wax amounts and composition from stems and leaves of 3- to 5-week-old Arabidopsis wild-type plants, which were grown under long-day conditions (16 h of light/8 h of dark; Control) and in the dark for 6 d (Dark). Cuticular waxes were extracted with chloroform and analyzed by GC-FID and GC-MS. Data were statistically analyzed using Student’s t test (*P < 0.01). Error bars indicate se from triplicate experiments. FA, fatty acids; AL, aldehydes; PA, primary alcohols; AK, alkanes; SA, secondary alcohols; KE, ketones.

(B) Expression of wax biosynthetic genes from stems and leaves of 3- to 5-week-old Arabidopsis wild-type plants, which were grown under long-day conditions (16 h of light/8 h of dark; Control) and in the dark for 6 d (Dark). Total RNA was extracted from each sample and subjected to qRT-PCR analysis. Values from plants grown in dark conditions were divided by those from plants grown in control conditions, and the data were analyzed using Student’s t test (*P < 0.01). Error bars indicate sd from biological triplicate experiments. nd, not detected. The y axis is shown in the logarithmic scale (10^∧).