Figure 2.

Dynamics of GFP-RbohD Foci at the Plasma Membrane in Arabidopsis Seedlings.

(A) A single-particle image of diffraction-limited spots of GFP-RbohD at the plasma membrane in living cells imaged with VA-TIRFM.

(B) Dynamic analysis using a time series of the boxed area in (A). Red and blue circles indicate the fluorescent spots analyzed in (C).

(C) Fluorescence intensity profile of the various foci indicated in (B).

(D) The corresponding kymograph from a representative time series recorded for 15 s.

Bars = 5 μm (A) and 2 μm (B).