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. 2014 Apr 22;26(4):1729–1745. doi: 10.1105/tpc.113.122358

Figure 3.

Figure 3.

Diffusion Coefficient Distribution, Fluorescence Intensity, and Oligomeric State Analysis of GFP-RbohD Foci at the Plasma Membrane.

(A) Distribution of diffusion coefficients (D) of GFP-RbohD spots on the epidermal cell plasma membrane (n = 526 spots).

(B) Distribution of fluorescence intensities of GFP-RbohD spots in living cells (n = 688 spots).

(C) Time courses of GFP emission after background correction, showing one-step bleaching.

(D) Time courses of GFP emission after background correction, showing two-step bleaching. The amplitude of each step was similar to that of single purified GFP molecules observed on cover slips (Supplemental Figure 2).