Figure 5.

Interruption of the Clathrin-Dependent Pathway Affects GFP-RbohD Foci Dynamics at the Plasma Membrane.

(A) VA-TIRFM image of epidermal cells fluorescently labeled with GFP-RbohD and treated with TyrA23. Bar = 5 μm.

(B) Distribution of GFP-RbohD diffusion coefficients on the epidermal cell plasma membrane in the presence of TyrA23 (n = 475 spots).

(C) VA-TIRFM image of GFP-RbohD in the chc2-1 mutant. Bar = 5 μm.

(D) Distribution of GFP-RbohD diffusion coefficients on the epidermal cell plasma membrane in the chc2-1 mutant (n = 430 spots).

(E) VA-TIRFM image of epidermal cells fluorescently labeled with GFP-RbohD and treated with TyrA51. Bar = 5 μm.

(F) Distribution of GFP-RbohD diffusion coefficients on the epidermal cell plasma membrane in the presence of TyrA51 (n = 612 spots).

[See online article for color version of this figure.]