Figure 7.

Effects of Sterol on the Localization and Diffusion of GFP-RbohD at the Plasma Membrane.

(A) Distribution of GFP-RbohD spots in control seedlings.

(B) Effect of mβCD on the distribution pattern of GFP-RbohD at the plasma membrane.

(C) Distribution of GFP-RbohD diffusion coefficients on the epidermal cell plasma membrane in the presence of mβCD (n = 429 spots).

(D) to (F) In the Ler control, GFP-RbohD was localized principally at the plasma membrane in the leaf (D), hypocotyl (E), and root (F).

(G) to (I) Confocal images of dry2/sqe1-5 mutants showing uneven GFP-RbohD distribution at the membrane in leaf (G), hypocotyl (H), and root (I).

Bars = 5 μm in (A) and (B) and 20 μm in (D) to (I).