Figure 9.

Effects of NaCl Treatment on the Localization and Dynamic Behavior of GFP-RbohD.

(A) In control cells, after pretreatment with cycloheximide (50 μM) for 30 min, distinct GFP-RbohD and FM4-64 colocalization was observed at the plasma membrane; a few spots were found in the cytoplasm. Bar = 20 μm.

(B) NaCl treatment led to the uneven distribution of GFP-RbohD at the plasma membrane. After pretreatment with cycloheximide (50 μM) for 30 min, GFP-RbohD colocalized with FM4-64 in the cytoplasm upon exposure to NaCl. White arrows indicate cytoplasmic fluorescence clusters. Bar = 20 μm.

(C) Dynamic partitioning of GFP-RbohD on the plasma membrane of epidermal cells in the presence of NaCl. Bar = 5 μm.

(D) Distribution of GFP-RbohD diffusion coefficients on the epidermal cell plasma membrane under NaCl stimulation (n = 633 spots).

(E) Distribution of diffusion coefficients upon treatment with mβCD prior to exposure to NaCl (n = 491 spots).

(F) Distribution of diffusion coefficients after pretreatment with TyrA23 prior to exposure to NaCl (n = 475 spots).