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. Author manuscript; available in PMC: 2015 Apr 18.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Mar 15;446(4):901–905. doi: 10.1016/j.bbrc.2014.03.032

Figure 3.

Figure 3

Whole N-terminus domain of EnoBs is necessary as a signal for the secretion of recombinant GFP. Series deletion of N-terminus from its C-terminal were prepared and fused with GFP. Recombinant GFPs were expressed in B. subtilis. Soluble Intracellular and Extracellular samples were analyzed for GFP (middle panel) were detected using immunoblots with antibodies. SecA (upper panel) was used as a marker for no secretion, and chromosomal enolase (lower panel) as a marker for expression and secretion. Lane 1, GFP; lane 2, N140-GFP; lane 3, N126-GFP; lane 4, N118-GFP; lane 5, N109-GFP; lane 6, #106–123Eno-GFP.