Table 1.

Percent photolysis of cobalamin-fluorophore conjugate upon illumination at 546 nm (5 min), 646 nm (5 min), 700/727 nm (20 min) and 777 nm (10 min). Percent photolysis was calculated using the maximal observed fluorescent increase as 100% photolysis (see Figs S8, S10, S12, S14, S17, and S24).

Cobalamin Conjugate	Fluorophore	λab/ex (nm) [a]	Percent Photolysis at Applied Wavelength (nm)
			546	646	700/727[b]	777
Cbl-1	TAMRA	546	100 ± 17	[c]	[c]	[c]
Cbl-3	SulfoCy5	546, 646*	13 ± 21	100 ± 21	[c]	[c]
Cbl-4	Atto725	546, 646, 727*, 777	62 ± 8	35 ± 5	100 ± 5	9 ± 5
Cbl-5	Dylight800	546, 727, 777*	22 ± 12	[c]	20 ± 12	100 ± 12
Cbl-6	Alexa700	546, 646, 700*	56 ± 3	48 ± 5	100 ± 3	[c]
Cbl-Bod	Bodipy650	546, 646*	48 ±12	100 ± 12	[c]	[c]

^[a]Absorbance/excitation of the cobalamin-fluorophore conjugate where * is the major fluorophore excitation band.

^[b]All compounds were photolyzed at 727 nm, except Cbl-6, which was photolyzed at 700 nm (3 min).

^[c]<5% photolyzed under the experimental conditions.