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. 2010 Jul 2;3(7):2045–2058. doi: 10.3390/ph3072045

Figure 2.

Figure 2

Effects of EGF treatment on EL4 cell proliferation. Equal numbers of EL4 cells (2 × 105) were plated and grown overnight. Cells were then serum starved for 24 hours. Serum-starved cells were treated with 10 nM EGF for 24 hours. Following treatment, viable cells were counted using hemacytometer. Cell numbers are presented as percent of control (without serum); each data point represents mean ± SD from three separate wells. Statistical significance was determined using t-test: * p < 0.05.