Figure 4.

Time course of the effects of EGF on Akt activation in EL4 cell lines. Cells were serum starved 24 hours prior to EGF treatment. Serum-starved cells were treated for indicated times with or without (“C”) 10 nM EGF. Equalized protein (100 μg) from whole-cell lysates was resolved by SDS-PAGE and transferred to a PVDF membrane. An antibody recognizing phospho-Akt was used to detect activated Akt. Anti-actin antibody was used as a loading control. The blot was developed using chemiluminescence reagents.