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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Cell Mol Bioeng. 2014 Mar 11;7(2):218–224. doi: 10.1007/s12195-014-0325-z

Figure 1. Oscillatory shear (OS) induces DNA hypermethylation in endothelial cells (ECs).

Figure 1

ECs were exposed to OS (0.5 ± 4 dynes/cm2) or PS (12±4 dynes/cm2) for 24 hr, and 5-meC level was determined by (A) immunofluorescent staining (images shown are representative from three independent experiments) and (B) Left: Immuno-slot blot using anti-5-meC. Right: The blot membrane was incubated with methylene blue to indicate equal loading of DNA.