Table 3.

Experimental design and results of CCD for enzymatic hydrolysis of pretreated Parthenium sp.

Run number	A: temperature (°C)	B: pH	C: enzyme loading (mL)	D: substrate loading (g)	Saccharification efficiency (%)
					Experimental	Predicted
1	50	4.25	0.8	0.2	72.52	75.16
2	60	4.25	0.4	0.4	17.38	20.40
3	50	4.75	0.4	0.4	46.88	39.52
4	55	4.5	0.6	0.3	54.73	54.73
5	60	4.25	0.8	0.2	25.97	29.81
6	50	4.25	0.4	0.2	61.51	57.78
7	55	4.5	0.6	0.1	35.67	36.52
8	60	4.25	0.8	0.4	23.21	24.78
9	55	4.5	1.0	0.3	71.52	64.83
10	50	4.25	0.8	0.4	59.14	56.79
11	45	4.5	0.6	0.3	68.50	75.31
12	60	4.75	0.8	0.2	22.16	28.07
13	55	4.5	0.2	0.3	28.41	37.07
14	55	4.5	0.6	0.3	54.73	54.73
15	50	4.75	0.8	0.2	83.27	76.73
16	60	4.75	0.8	0.4	23.67	23.87
17	60	4.75	0.4	0.2	15.15	13.98
18	60	4.25	0.4	0.2	26.29	21.71
19	65	4.5	0.6	0.3	8.78	3.94
20	55	4.5	0.6	0.5	16.52	17.66
21	55	5.0	0.6	0.3	38.12	39.10
22	55	4.0	0.6	0.3	43.45	44.44
23	55	4.5	0.6	0.3	54.73	54.73
24	50	4.75	0.4	0.2	53.37	53.35
25	55	4.5	0.6	0.3	54.73	54.73
26	55	4.5	0.6	0.3	54.73	54.73
27	50	4.75	0.8	0.4	53.05	59.18
28	50	4.25	0.4	0.4	47.47	43.12
29	60	4.75	0.4	0.4	14.59	13.50
30	55	4.5	0.6	0.3	54.73	54.73