Table 4.
ANOVA for quadratic response surface model (RSM) from enzymatic saccharification of pretreated Parthenium sp.
| Source | Sum of squares | Degree of freedom | Mean square | F value | P value (Prob > F) |
|---|---|---|---|---|---|
| Model | 11340.56 | 14 | 810.04 | 26.51 | <0.0001 |
| A: temperature | 7640.87 | 1 | 7640.87 | 250.04 | <0.0001 |
| B: pH | 42.69 | 1 | 42.69 | 1.40 | 0.2556 |
| C: enzyme loading | 1155.07 | 1 | 1155.07 | 37.83 | <0.0001 |
| D: substrate loading | 533.46 | 1 | 533.46 | 17.45 | 0.0008 |
| AB | 10.91 | 1 | 10.91 | 0.36 | 0.5591 |
| AC | 86.26 | 1 | 86.26 | 2.82 | 0.1136 |
| AD | 178.29 | 1 | 178.29 | 5.83 | 0.0289 |
| BC | 35.91 | 1 | 35.91 | 1.18 | 0.2955 |
| BD | 0.69 | 1 | 0.69 | 0.023 | 0.8823 |
| CD | 13.78 | 1 | 13.78 | 0.45 | 0.5121 |
| A 2 | 391.03 | 1 | 391.03 | 12.80 | 0.0028 |
| B 2 | 287.84 | 1 | 287.84 | 9.42 | 0.0078 |
| C 2 | 24.47 | 1 | 24.47 | 0.80 | 0.3850 |
| D 2 | 1310.41 | 1 | 1310.41 | 42.88 | <0.0001 |
| Residual | 458.38 | 15 | 30.56 | ||
| Lack of fit | 458.38 | 10 | 45.84 | ||
| Pure error | 0.00 | 5 | 0.00 | ||
| Total | 11798.95 | 29 | |||
|
| |||||
| SD | 5.53 | R 2 | 0.96 | ||
| Mean | 42.83 | Adjusted R 2 | 0.93 | ||
| CV(%) | 12.91 | Predicted R 2 | 0.78 | ||