Table 1.
Species specific primers used in our study.
| Name of bacteria | Forward primer (5′-3′) | Reverse primer (5′-3′) | T a** (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| Pg | TGTAGATGACTGATGGTGAAAACC | ACGTCATCCCCACCTTCCTC | 60 | 197 |
| Tf | GCGTATGTAACCTGCCCGCA | TGCTTCAGTGTCAGTTATACCT | 60 | 641 |
| Pi | TTTGTTGGGGAGTAAAGCGGG | TCAACATCTCTGTATCCTGCGT | 55 | 575 |
| Pn | ATGAAACAAAGGTTTTCCGGTAAG | CCCACGTCTCTGTGGGCTGCGA | 55 | 804 |
| Cr | TTTCGGAGCGTAAACTCCTTTTC | TTTCTGCAAGCAGACACTCTT | 60 | 598 |
| Ec | CTAATACCGCATACGTCCTAAG | CTACTAAGCAATCAAGTTGCCC | 60 | 688 |
| Aa | AGAGTTTGATCCTGGCTCAG | CACTTAAAGGTCCGCCTACGTGCC | 60* | 593 |
| Co | AGAGTTTGATCCTGGCTCAG | GATGCCGTCCCTATATACGGGG | 55 | 185 |
| Cs | AGAGTTTGATCCTGGCTCAG | GATGCCGCTCCTATATACCATTAGG | 55* | 185 |
| Td | AAGGCGGTAGAGCCGCTCA | AGCCGCTGTCGAAAAGCCCA | 55 | 311 |
*Confirmation was done using a separate run of PCR to differentiate from other bacteria in the same batch of PCR producing similar size of amplicons.
**T a: Annealing temperature.