Phylogenetic trees of the viral protein 1 (VP1) of bufaviruses, constructed by using deduced amino acid sequences by neighbor-joining method. The full length open reading frames of VP1 genes were used to deduce amino acid sequences. Porcine parvovirus strain NC 001718 was used as an out-group. The numbers adjacent to the nodes represent the bootstrap values, and values <50% are not shown. Scale bar indicates the genetic distances expressed as amino acid substitutions per site. Accession numbers for DNA Data Bank of Japan, European Molecular Biology Laboratory, and GenBank are AB847987, AB847988, and AB847989 for strains BTN-63, BTN-109, and BTN-310, respectively.