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. 2014 Jun;20(6):1075–1077. doi: 10.3201/eid2006.131596

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Phylogenetic analysis of enterovirus genotype C117 (EV-C117) based on nucleotide sequences. Phylogenetic trees were generated with 1,000 bootstrap replicates. Neighbor-joining analysis of the targeted nucleotide sequence was performed by using the Kimura 2-parameter model with Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 (http://www.megasoftware.net). The EV-C117 strains identified in this study are indicated by black circles. Enterovirus 68, cocksackievirus (CV) A2, and echovirus (E) 3 (GenBank accession nos. AY426531, AY421760, and AY302553) were used as outgroups. PV, poliovirus. A) Phylogenetic analysis of the VP 4/VP2 region (399 nt, corresponding to nt 673–1,071 of EV-C117 prototype strain LIT22 [JX262382]). B) Phylogenetic analysis of the viral protein1 region (888 nt, corresponding to nt 2416–3303, numbered according to the sequence of LIT22). Scale bars represent nucleotide substitutions per site.