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. 2007 Mar 30;133(1):63–73. doi: 10.1016/j.virusres.2007.02.015

Fig. 3.

Fig. 3

SDS-PAGE analysis of purified SARS-CoV 3CLpro constructs. The untagged version of the enzyme has a predicted molecular weight of 33.8 kDa. The (His)6-tagged construct, accordingly, is shifted to a slightly higher molecular. The native enzyme readily crystallizes in space group C2 (inset picture) when covalently modified by an inhibitor (Ghosh et al., 2005). In contrast, attempts to crystallize the (His)6-tagged construct in either its native or covalently modified form have been unsuccessful.