Figure 4.

Protein search tool comparison. Five methods of detecting KIFs were compared. Twenty-eight clones were detected by all five methods. Pfam and InterPro had low false positive and high false negative rates. Auto-annotation detected the most KIFs but also the most false positives. The false positives were greatly reduced from 18 to 8 by human annotation. Clones identified by respective number of search tools are indicated by the following colors: (yellow) all 5search tools, (green) 4 search tools; (red) 3 search tools; (white) 2 search tools; (blue) 1 seach tool; (black) false positive.