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letter
. 2003 Sep;13(9):2069–2081. doi: 10.1101/gr.1306003

Figure 3.

Figure 3

Patterns of endogenous Gdf6 gene expression are recapitulated by Gdf6-LacZ BAC transgenes. X-gal stained transgenic embryos are shown in comparison to cryostat sections of 14.5-dpc nontransgenic embryos hybridized with sense or antisense DIG-labeled riboprobes from the mouse Gdf6 gene. For Gdf6 antisense sections, arrowheads indicate positive staining relative to control in situ hybridizations with sense probes. (a) Whole-mount of X-gal-stained and cleared elbow from transgenic 15.5-dpc embryo forelimb. (b) Section through elbow region of nontransgenic embryo hybridized with Gdf6 antisense probe. Arrowheads indicate transgenic LacZ (a) or endogenous Gdf6 (b) expression at distal end of humerus. (c–e) Sagittal sections through skull basosphenoid cartilage. For (c–e), left is anterior and top is ventral. (c) Cryosection through X-gal-stained transgenic 14.5-dpc embryo showing stripe of LacZ expression in connective tissue just posterior to basosphenoid. (d) Section through nontransgenic embryo hybridized with Gdf6 antisense probe. (e) Near-adjacent section to d hybridized with sense probe. (f–h) Sagittal sections through larynx; top of panel is anterior and right is ventral. (f) Cryosection through X-gal stained transgenic 14.5-dpc embryo. LacZ staining is in connective tissue generally dorsal and adjacent to thyroid cartilage. (g) Section through nontransgenic embryo hybridized with Gdf6 antisense probe. (h) Near-adjacent section to g hybridized with sense probe. (i–k) Sagittal or near-sagittal sections through distal digit tips; for i–k, top of panel is dorsal and bottom is ventral. (i) Cryosection through X-gal-stained transgenic 12.5-dpc embryo. Note LacZ staining in ventral ectoderm is stronger distally but appears restricted to ventral ectoderm. (j) Near-sagittal section through nontransgenic embryo hybridized with Gdf6 antisense probe. (k) Near-adjacent section to j hybridized with sense probe. (l–n) Sagittal sections through distal jaws to reveal incisor buds; top of panel is anterior and right is ventral. (l) Whole-mount transgenic embryo bisected sagittally to reveal strong X-gal staining in dental epithelium of incisor buds. (m) Section through nontransgenic embryo hybridized with Gdf6 antisense probe. (n) Near-adjacent section to m hybridized with sense probe. bs, basosphenoid; sp, secondary palate; ph, pharynx; tr, trachea; th, thyroid cartilage; hy, hyoid cartilage; to, tongue; mn, mandible.