Table 3.

Importance of Protocols and Vectors for the Gene Discovery

A: Protocols	3′ Sequences	3′ Clusters	3′ Singletons	3′ Group specific clusters	3′ coding	5′ Sequences	5′ Clusters	5′ Singletons	5′ Group specific clusters	5′ coding
Normization and subtraction	772,209	61,732	47,509	21,947	96.14	140,707	30,911	61,555	7528	79.92
Small-scale	372,132	42,316	29,322	8238	96.51	228,086	32,249	31,851	7878	91.41
Standard	259,267	33,119	19,421	3943	96.65	78,407	18,883	15,779	1668	90.57
Normalization	168,383	21,178	12,057	1389	97.87	130,004	22,825	14,036	3252	89.97
Subtraction	157,238	27,792	11,799	3625	95.63	79,027	16,526	12,011	3444	91.17
Cytoplasmic RNA	144,752	21,348	7167	947	97.8	96,967	21,278	10,675	2347	90.15
Library subtraction	65,223	13,092	4710	577	98.23	94,781	17,012	10,037	2311	92.02
Size-selection, normalization and subtraction	59,750	16,837	2983	352	98	8698	3639	4184	146	77.13
Size-selection	22,342	7870	1241	102	96.76	5414	2068	1854	110	84.42
(details unavailable)	19,760	6556	920	222	95.68	2718	1472	652	67	86.01
Capfinder/SMART	156	133	21	0	(82)	954	589	280	12	75.05
Size-selection-normalization	35	17	1	0	(100)	29	13	2	1	(100)
B: Vectors
λ FLCI	1,178,336	70,945	84,780	46,073	96.59	481,725	48,407	103,631	34,504	89.09
λ ZAP	190,064	20,775	6458	2328	96.24	19,500	7559	5927	467	83.07
λ FLCII	48,656	12,287	3425	997	96.45	13,571	6174	3081	354	84.95
λ FLCIII	24,931	7128	1747	303	96.74	10,763	4395	1421	147	91.82
λ TripIEx	156	133	21	0	(82)	954	589	280	12	75.05
pBS	93	71	6	0	93.55	85	62	10	1	85.45