Table 5.
Use of Polyadenylation Signals Varies Depending on the Class of Expression
| Polyadenylation signals | AATAAA % | ATTAAA % | AATTAA % | AAATAA % | AGTAAA % | AATATA % | CATAAA % | TAATAA % | AATAAT % | Others % | Total with signals | No signals | Total sequences |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total 3′ Est collection | 71.377 | 16.872 | 0.948 | 0.997 | 2.830 | 2.076 | 1.818 | 0.419 | 0.191 | 2.473 | 1,128,930 | 313,306 | 1,442,236 |
| 3′ clusters of 2 or more | 72.000 | 16.801 | 0.891 | 0.912 | 2.763 | 1.977 | 1.731 | 0.380 | 0.170 | 2.377 | 1,083,955 | 261,843 | 1,345,798 |
| 3′ singletons | 56.371 | 18.604 | 2.324 | 3.046 | 4.451 | 4.465 | 3.920 | 1.339 | 0.691 | 4.789 | 44,975 | 51,463 | 96,438 |
| Standard libraries | 74.749 | 15.130 | 0.642 | 0.785 | 2.763 | 1.754 | 1.587 | 0.376 | 0.165 | 2.051 | 207,626 | 55,442 | 263,068 |
| Mild subtr/norm (5 ≤ RoT ≤ 50) | 73.830 | 15.718 | 0.462 | 0.779 | 3.021 | 1.700 | 1.866 | 0.370 | 0.165 | 2.089 | 167,664 | 36,294 | 203,958 |
| Strong subt/norm (200 > RoT > 50) | 69.613 | 18.003 | 1.000 | 0.995 | 2.697 | 2.218 | 1.721 | 0.407 | 0.199 | 3.148 | 202,835 | 41,692 | 244,527 |
| Very strong subt/norm (RoT ≥ 200) | 68.960 | 17.890 | 1.370 | 1.250 | 2.758 | 2.422 | 2.071 | 0.456 | 0.221 | 2.599 | 446,662 | 158,715 | 605,377 |
| Other protocols | 74.466 | 15.809 | 0.391 | 0.681 | 3.381 | 1.512 | 1.568 | 0.520 | 0.120 | 1.552 | 54,778 | 12,527 | 67,305 |
| Library conditions unavailable | 74.551 | 15.446 | 0.464 | 0.689 | 3.061 | 1.612 | 1.019 | 0.357 | 0.152 | 2.650 | 49,365 | 8636 | 58,001 |