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(Protein samples were denatured by heating for 5 min in the presence of SDS and β-mercaptoethanol): Lane 1: Protein markers, Lane 2: Acetone precipitated proteins, Lane 3: Sephadex-150 Column purified enzyme, lane 4: DEAE-Cellulose anion exchange Column purified enzyme, Lane 5: Activity staining; purified laccase stained with guaiacol (samples were heated for 5 min in the presence of SDS without β-mercaptoethanol).
