Table 3. Kinetic properties of purified SN4LAC in comparison to other laccases.
Substrates | Bacillus tequilensis SN4 | Bacillus sp. HR03 [23] | Streptomyces ipomea [8] | ||||||
K m (mM) | k cat (s−1) | k cat/K m (mM−1 s−1) | K m (mM) | k cat (s−1) | k cat/K m (mM−1 s−1) | K m (mM) | k cat (s−1) | k cat/K m (mM−1 s−1) | |
ABTS | 1.404±0.08 | 67.04 | 47.82 | 0.535 | 127 | 237 | 0.4 | 9.99 | 24.975 |
DMP | 0.840±0.012 | 73.15 | 87.05 | 0.053 | 3 | 56.60 | 4.2 | 4.20 | 1 |
Guaiacol | 3.258±0.096 | 62.96 | 19.34 | - | - | - | - | - | - |
Values represent mean ± SD (n = 3).