Table 4. Effect of inhibitors on SN4LAC activity.
Inhibitors | Conc. (mM) | Relative activity (%) |
Control | ---- | 100 |
Sodium azide | 1 | 76.95±3.82 |
5 | 53.86±1.40 | |
10 | 32.57±1.22 | |
DTPA | 1 | 33.99±.66 |
5 | 31.82±0.72 | |
10 | 23.81±1.06 | |
EDTA | 1 | 42.45±1.03 |
5 | 27.83±0.18 | |
10 | 21.53±1.18 | |
Cysteine | 1 | 63.75±1.65 |
5 | 1.84±0.05 | |
10 | 1.45±0.05 | |
Dithiothreitol | 1 | 20.56±1.28 |
5 | 0 | |
10 | 0 | |
B-mercaptoethanol | 1 | 0 |
5 | 0 | |
10 | 0 |
Values represent mean ± SD (n = 3) relative to untreated control sample.