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. 2003 Oct;13(10):2341–2347. doi: 10.1101/gr.1478703

Figure 1.

Figure 1

The RNAi microarray platform. (A) HeLa cells expressing a destabilized version of eGFP (HeLa/d2eGFP) cells were plated on slides arrayed with an egfp siRNA (2.5 ng) tagged with fluorescent rhodamine (rh-egfp). HeLa/d2eGFP cells grown on an rh-egfp siRNA microarray slide for 72 h were fixed with 2% paraformaldehyde and counterstained with DAPI. (B) An rh-egfp spot (2.5 ng siRNA) showing the localized inhibition of green fluorescence (25×, green channel only). (C) The uptake of rh-egfp siRNA-lipoplexes showing cellular accumulation of the siRNA in silenced cells (1000×). (D) A clear edge to the arrayed rh-egfp siRNA spot was present only in the green channel (200×, overlaid green and blue images), with (E) inhibition of eGFP fluorescence occurring within the spot (400×, overlaid green and blue images). (F) No inhibition of eGFP fluorescence in cells was observed around the rh-egfp siRNA spot (400×, overlaid green and blue images).