Effect of conditioned medium (CM) of the aged MDPSCs compared with those of aged DPSCs, young MDPSCs and young DPSCs. (A) The proliferative effect in NIH3T3 cells. (*p<0.05, young MDPSCs CM versus young DPSCs CM; S
p<0.05, aged MDPSCs CM versus aged DPSCs CM). (B) The migratory effect in NIH3T3 cells (*p<0.05, **p<0.01, young MDPSCs CM versus young DPSCs CM; S
p<0.05, SS
p<0.01, aged MDPSCs CM versus aged DPSCs CM; #
p<0.05, young DPSCs CM versus aged DPSCs CM). (C) Mixed lymphocyte reaction (MLR) analysis. (*p<0.05, **p<0.01, young MDPSCs CM versus young DPSCs CM; SS
p<0.01, aged MDPSCs CM versus aged DPSCs CM; #
p<0.05, ##
p<0.01, young DPSCs CM versus aged DPSCs CM). (D) The relative percentage of viable and apoptotic cells analyzed by flow cytometry by Annexin V staining. *p<0.05, **p<0.01. Data are expressed as the means ± SD of 6 determinations. The experiments were repeated three times (6 lots), and one representative experiment is presented.