Table 3.
Precision and accuracy of quality control samples
| Analyte | Nominal concentration (ng/mL) |
Accuracy (%) | Precision (%CV) | |||||
|---|---|---|---|---|---|---|---|---|
| Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 | Inter-day | ||
| Equol | 50 | 100.42 | 90.13 | 96.60 | 2.01 | 4.33 | 5.11 | 3.74 |
| 500 | 103.30 | 99.85 | 114.66 | 2.31 | 5.61 | 1.93 | 2.97 | |
| 2,000 | 97.60 | 89.90 | 103.96 | 6.11 | 10.61 | 10.13 | 8.34 | |
| Daidzein | 50 | 99.98 | 102.73 | 94.04 | 4.35 | 6.44 | 8.23 | 6.62 |
| 500 | 101.48 | 98.31 | 97.73 | 3.14 | 5.44 | 7.42 | 5.38 | |
| 2,000 | 92.50 | 87.41 | 86.03 | 2.88 | 3.61 | 3.96 | 3.58 | |
| Dihydrodaidzein | 50 | 103.00 | 100.15 | 101.66 | 3.94 | 1.43 | 4.99 | 3.63 |
| 500 | 103.79 | 95.20 | 106.00 | 3.96 | 6.44 | 3.35 | 4.34 | |
| 2,000 | 91.70 | 90.40 | 96.33 | 1.68 | 5.80 | 6.60 | 2.82 | |
| O-DMA | 50 | 104.00 | 93.72 | 96.51 | 5.16 | 4.71 | 5.80 | 5.32 |
| 500 | 105.67 | 93.78 | 102.33 | 3.22 | 9.42 | 5.54 | 5.84 | |
| 2,000 | 101.20 | 93.57 | 100.93 | 5.53 | 5.37 | 6.53 | 3.63 | |
| Genistein | 50 | 107.66 | 106.83 | 99.08 | 3.97 | 3.37 | 6.65 | 4.86 |
| 500 | 97.50 | 88.90 | 91.36 | 5.40 | 3.61 | 5.60 | 4.96 | |
| 2,000 | 95.13 | 92.28 | 93.38 | 2.63 | 3.97 | 4.17 | 3.59 | |
| Glycitein | 50 | 97.00 | 80.00 | 92.13 | 11.64 | 7.77 | 7.52 | 8.59 |
| 500 | 100.66 | 91.96 | 99.96 | 6.76 | 5.09 | 5.24 | 5.58 | |
| 2,000 | 96.20 | 92.70 | 100.21 | 2.34 | 5.87 | 5.19 | 4.29 | |
| Formononetin | 50 | 95.43 | 98.65 | 98.50 | 4.28 | 7.69 | 4.93 | 5.59 |
| 500 | 101.53 | 96.60 | 108.66 | 4.95 | 6.09 | 2.99 | 4.35 | |
| 2,000 | 92.72 | 94.08 | 101.90 | 2.09 | 7.80 | 2.26 | 3.78 | |
| Coumestrol | 50 | 92.97 | 89.24 | 99.42 | 8.66 | 9.15 | 6.69 | 7.66 |
| 500 | 93.86 | 93.95 | 96.03 | 9.05 | 7.78 | 5.34 | 7.26 | |
| 2,000 | 100.45 | 89.95 | 84.12 | 7.83 | 7.34 | 3.66 | 6.95 | |
| Biochanin A | 50 | 104.23 | 103.13 | 107.50 | 6.82 | 3.88 | 3.26 | 4.48 |
| 500 | 89.95 | 94.01 | 98.41 | 4.46 | 6.43 | 5.24 | 5.15 | |
| 2,000 | 98.88 | 95.00 | 93.68 | 2.91 | 3.10 | 5.47 | 3.90 | |
| Enterodiol | 50 | 93.35 | 88.63 | 96.95 | 9.22 | 7.46 | 5.36 | 7.11 |
| 500 | 104.95 | 98.95 | 112.16 | 7.81 | 4.10 | 4.12 | 4.98 | |
| 2,000 | 96.50 | 87.40 | 94.73 | 3.98 | 6.05 | 7.94 | 5.86 | |
| Enterolactone | 50 | 104.15 | 102.20 | 108.83 | 9.23 | 5.71 | 2.50 | 5.57 |
| 500 | 103.30 | 92.26 | 104.00 | 5.43 | 8.33 | 1.98 | 5.03 | |
| 2,000 | 84.68 | 83.33 | 91.90 | 1.52 | 0.35 | 7.56 | 3.09 | |