| Tfb I (Transformation Buffer I) | |||
|---|---|---|---|
| Component | Final concentration | Stock | Amount/400 ml |
| Potassium acetate | 30 mM | - | 1.18 g |
| RbCl2 | 100 mM | - | 4.84 g |
| CaCl2 -2H2O | 10 mM | - | 0.59 g |
| MnCl2 | 50 mM | - | 3.96 g |
| glycerol | 15% v/v | - | 60 ml |
| pH 5.8 with dilute acetic acid. Add water to 400 ml and filter sterilize | |||
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.