| Tfb II (Transformation Buffer II) | |||
|---|---|---|---|
| Component | Final concentration | Stock | Amount/100 ml |
| MOPS | 10 mM | - | 0.21 g |
| CaCl2-2H2O | 75 mM | - | 1.1 g |
| RbCl2 | 10 mM | - | 0.12 g |
| Glycerol | 15% v/v | - | 15 mls |
| pH 6.5 with dilute NaOH. Add water to 100 ml and filter sterilize | |||
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