| Step 2 | |
|---|---|
| LB Agar (Miller’s high salt) | |
| Component | Amount/liter |
| Tryptone | 10 g |
| Yeast Extract | 5 g |
| NaCl | 10 g |
| Agar | 15 g |
| Adjust to pH 7.2 (~0.2 ml of 5 N NaOH) Add water to 1 liter and autoclave, add appropriate antibiotic when cool and pour plates | |
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