SOC media
Component	Final concentration	Stock	Amount/100 mls
Yeast extract	0.5%	-	0.5 g
Tryptone	2%	-	2.0 g
NaCl	10 mM	3 M	0.33 ml
KCl	2.5 mM	1 M	0.25 ml
MgCl2	10 mM	1 M	1 ml
MgSO4	10 mM	1 M	1 ml
Glucose	20 mM	1.1 M	1.82 ml
Add water to 100 mls aliquot in 5–10 ml samples and autoclave