Duration
	Preparation	about 15 minutes
	Protocol	about 4–6 hours
Preparation	Make sure you have selective agar plates on hand.
	Pick a single colony from a freshly streaked plate and inoculate a small culture (2–5 mls). Grow overnight at 37°C.
	TfbI and TfbII should be stored at 4°C to make sure they are chilled