Skip to main content
. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Stem Cells. 2014 Jun;32(6):1480–1492. doi: 10.1002/stem.1667

Figure 6. Comparative RNAseq analysis of WT and (R200Q)VSX2 hiPSC-OVs: differences in expression of genes involved in early retinal differentiation.

Figure 6

(R200Q)VSX2 mutant hiPSC-OVs were subjected to high throughput transcriptome analysis. Differential expression analysis performed with GeneSifter software revealed the total number of genes significantly changed (p < 0.05) in mutant hiPSC-OVs compared to WT at day 20 (A) and day 30 (B). At day 30, the majority of such genes were upregulated in mutant hiPSCs-OVs, consistent with a transcriptional repressor function for VSX2. Expression levels of selected genes involved in retinal differentiation were also compared between the two groups at day 20 (C) and day 30 (D), with genes up- or downregulated in mutant hiPSC-OVs highlighted in red or green, respectively. Unchanged genes are highlighted in blue. MITF, NR2F2, OTX1, and OTX2, transcription factors involved in RPE development, were upregulated in mutant hiPSC-OVs at day 30. TPM=transcripts per million, SEM=standard error of the mean.