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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Stem Cells. 2014 Jun;32(6):1649–1660. doi: 10.1002/stem.1659

Figure 1. HG primes MPCs to alter their differentiation potential through selective modulation of Wnt signaling.

Figure 1

(A) Schematic showing treatment groups for the HG priming study [MPC = mesenchymal progenitor cell; HG = 25 mmol/L glucose; Adipo = adipogenic induction media]. (B) Extent of adipogenesis was measured by induction of adipogenesis-specific transcription factors: c/EBPβ and c/EBPδ (early transcription factors), c/EBPα and PPARγ2 (late transcription factors), and FABP4 (marker for differentiated adipocytes) [data are represented as mean ± SEM; *p<0.05 compared to adipogenic media (Adipo)]. (C) MPCs were differentiated into adipocytes in the presence or absence of HG and mRNA expression of Wnt ligands was examined [data are represented as mean ± SEM; *p<0.05 compared to Control]. (D) Wnt11 mRNA levels in MPCs after treatment with HG for 7 days in normal growth media (DMEM/10% FBS) [data are represented as mean ± SEM; *p<0.05 compared to Control (DMEM/10% media with 5 mmol/L glucose)]. (E) mRNA levels of frizzled receptors and secreted frizzled-related proteins (SFRP-1 and -4) [data are represented as mean ± SEM; *p<0.05 compared to Control]. (F) mRNA expression of β-catenin, negative transcriptional regulators of canonical Wnt signaling (Transducin-Like Enhancer Proteins 1/2,TLE1/2; β-catenin interacting protein-1,CTNNBIP1), and downstream target genes (CyclinD1/D2/D3; Myc; Wnt-induced secreted protein-1, WISP1) in control, Adipo and Adipo + HG groups [data are represented as mean ± SEM; *p<0.05 compared to Control].