Table 2.
Most Abundant Striatal Markers
|
Tag count
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Marker | Identification | Tag sequence | Whole brain | Lateral striatum | Medial striatum | Nucleus accumbens | Somatosensory cortex | Kidney | Embryo |
| StM1 | Phosphodiesterase 1B, Ca2+-calmodulin-dependent, 63 kD (L01695) | AATAAAGAAG | 5 | 58* | 35* | 8 | 4 | 0 | 1 |
| TAGATGGGTTa | 0 | 5* | 4 | 1 | 1 | 0 | 0 | ||
| StM2 | Proenkephalin (M13227) | TGCTGAAAGA | 4 | 34* | 32* | 21* | 2 | 0 | 0 |
| StM3 | ESTs (BE993937) | GAATGCCATT | 4 | 21* | 35* | 2 | 6 | 0 | 0 |
| StM4 | Cyclic AMP-regulated phosphoprotein, 21 kD (AK002950) | TCAGACCCAC | 4 | 16 | 22* | 14 | 1 | 0 | 0 |
| StM5 | Calcineurin catalytic subunit, α isoform (J05479, BQ176158) | ACTAGCTTCG | 4 | 24* | 12 | 4 | 15 | 0 | 0 |
| AAAGCATGCAa | 19 | 47 | 53 | 7 | 30 | 0 | 0 | ||
| StM6 | ESTs (AA030405) | TCTGTGGCCT | 2 | 20* | 15* | 14 | 3 | 0 | 1 |
| StM7 | Striatum-specific G-protein coupled receptor 88 (AB042408) | CCAGTGGAGA | 0 | 12* | 19* | 2 | 1 | 0 | 0 |
| StM8 | Striatum-enriched protein tyrosine phosphatase, 61 kD (U28217) | TGAGCCCTGA | 2 | 17* | 13* | 6 | 1 | 1 | 0 |
| StM9 | Kv channel-interacting protein 2 (AI851528) | CAATAAAACA | 0 | 11* | 10* | 2 | 2 | 0 | 0 |
| StM10 | No reliable match | TCTGGCTATA | 1 | 15* | 6* | 3 | 1 | 0 | 0 |
| StM11 | Tescalcin (AF234783) | TGGCAGGGCA | 2 | 14* | 7 | 3 | 1 | 2 | 0 |
| StM12 | Rap1 GTPase-activating protein 1 (AK019076) | ACTGAAGCCT | 1 | 7 | 13* | 4 | 2 | 3 | 0 |
| StM13 | α2, 8-sialyltransferase ST8SiaIII (X80502) | AGAATCTTCA | 1 | 10* | 10* | 1 | 5 | 0 | 0 |
| StM14 | Focal adhesion kinase 2 (BE996553) | GGTGGTTGAA | 3 | 7 | 10* | 1 | 2 | 0 | 0 |
| Stm15 | Ras homolog enriched in striatum (BC026377) | ATGTTACATA | 2 | 11* | 5 | 1 | 1 | 0 | 0 |
| StM16 | Preprotachykinin A (D17584) | TCTCACAAAA | 0 | 9* | 6* | 5* | 0 | 0 | 0 |
| StM17 | Dopamine and cyclic AMP-regulated phosphoprotein, 32 kD (BC011122) | TCCTCCTTCC | 1 | 9* | 5 | 4 | 0 | 9 | 0 |
| CGGCGCCGGAa | 0 | 5* | 7* | 2 | 0 | 0 | 0 | ||
| StM18 | Synaptoporin (AK005132) | TGTGATGTTG | 3 | 2 | 12* | 2 | 4 | 0 | 0 |
| StM19 | Regulator of G-protein signaling 9, alternatively spliced (AF125046) | AAAATGGAAA | 0 | 5* | 8* | 1 | 0 | 0 | 0 |
| StM20 | ESTs (AI414870) | AAGAACCTGG | 0 | 6* | 6* | 1 | 1 | 0 | 0 |
| StM21 | Adenylyl cyclase 5 (BC035550) | AAGACCATAG | 0 | 2 | 10* | 0 | 0 | 0 | 0 |
| StM22 | G-protein, γ 7 subunit (BC034108) | TCCGTGAATT | 0 | 7* | 5* | 1 | 0 | 0 | 0 |
| StM23 | cDNA sequence AK002772 | TCTGTATGTT | 1 | 10* | 2 | 3 | 1 | 0 | 1 |
| StM24 | Dopamine receptor 2 (X55674) | AGGTGGTAGA | 0 | 5* | 6* | 4 | 0 | 0 | 0 |
| StM25 | Anaphase-promoting complex, subunit 5 (AK012579)b | AGTTTGTCTG | 0 | 5* | 6* | 1 | 1 | 2 | 5 |
| StM26 | Testican 3 (AJ278998) | TTGGAATCAT | 1 | 6* | 5 | 2 | 1 | 0 | 0 |
| StM27 | ESTs (AI843433)b | ATTAAATGTA | 1 | 8* | 2 | 0 | 0 | 0 | 0 |
| StM28 | No reliable match | CTCAGTGGAT | 1 | 8* | 2 | 0 | 0 | 0 | 0 |
| StM29 | Hippocalcin (AK002992) | TACTTCCCTG | 1 | 6* | 4 | 0 | 2 | 0 | 0 |
| StM30 | Next to the BRCA1 (U73039)b | TTAATTACTC | 0 | 5* | 5* | 2 | 0 | 1 | 1 |
| StM31 | Ca2+-independent receptor of α latrotoxin, 1 (BC026388) | TTTCTCTAGG | 1 | 2 | 8* | 1 | 2 | 0 | 2 |
Markers are displayed in decreasing order of the corresponding tag count in both striatal libraries, from n = 93 to n = 10. These 10 nucleotide tags are located immediately downstream the most 3′ Sau3Al site of each polyadenylated transcript. Novel markers validated by in situ hybridization or real-time quantitative RT-PCR are shown in bold. Fulfillment of the criteria for region specificity is indicated by an asterisk. Data on mouse kidney and embryo are from Virlon et al. (1999) and Zakin et al. (2000)
a
More than one tag matched the same gene
b
Putative identification, not supported by in situ hybridization or quantitative RT-PCR