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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Mar 11;72:56–63. doi: 10.1016/j.yjmcc.2014.02.019

Figure 2. NADPH oxidase, SERCA oxidation and VCAM1 are increased in smooth muscle cells (SMCs) from HFHSD-fed mice.

Figure 2

A. mRNA levels of indicated genes in SMCs isolated from aortas of HFHSD- or ND-fed mice and cultured in 0.2% FBS DMEM overnight. *p < 0.05 vs. ND, n=5. B. Representative Western blots of homogenates of aortic SMCs isolated from HFHSD- or ND-fed mice and cultured in 0.2% FBS DMEM overnight. Nox2 (58 kDa), Rac1 (21 kDa), Nox4 (66 kDa), SERCA and SERCA C674-SO3H (110 kDa), p65NFκB and p-p65NFκB (65 kDa), VCAM1 (100 kDa), TNFα (17 kDa), GAPDH (36 kDa). Quantitation of band intensities in graph. *p < 0.05 vs. ND, n=3–10.