Figure 4. Downregulation of Nox2 restores NO function and inhibits inflammation in HFHSD SMCs.
A. Nox2 knockdown by siRNA decreases irreversible SERCA oxidation and the expression of inflammatory molecules. *p < 0.05 vs. control siRNA, n=3–7. B. Nox2 siRNA has no effects on other NADPH oxidase components mRNA. *p < 0.05 vs. control siRNA, n=3–6. C. Nox2 siRNA restored the inhibition of SMC migration by NO donor DETA NONOate. *p < 0.05 vs. control siRNA, n=6. D. Nox2 knockdown reduces macrophage adhesion to cultured SMCs isolated from HFHSD-fed mice. *p < 0.05 vs. control siRNA, n=4.