Figure 1. A. phagocytophilum infects granulocytes at differing efficiencies.
DNA was extracted from cells at 24 and 48 h for PMNs and 72 h for other cells. The DNA was analyzed by qPCR for the presence of A. phagocytophilum infection with msp2/B-actin 5’ nuclease assay and the number of A. phagocytophilum per cell was determined. Infection was performed in quadruplicate for neutrophils and triplicate for remaining cells. The mean ± SEM for 24 and 48 h infected neutrophils and 72 h time point for all other cell lines is displayed.