Figure 3. ranulocyte oxidative burst is decreased with A. phagocytophilum infection.

Intracellular levels of reactive oxygen species in response to the stimulant PMA were detected using DCFH-DA 72 h post-infection for cell lines and HSCs and 24 h post-infection for neutrophils. The relative fluorescence at the 2 h time point was plotted for undifferentiated (A) and differentiated (B) cells with (Aph) and without infection. Infections were repeated in triplicate and each sample was read in duplicate. Mean ± SEM is plotted.