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. 2003 Jul;13(7):1665–1674. doi: 10.1101/gr.288103

Table 1.

Mutation Sequence Data

TP ORF Number of base calls from 5′ end of gene Result Number of base calls from 3′ end of gene Result
11 651 696 error type 2a
13b 592 error type 3c
74 664 error type 3c 325 error type 2a
117 874 678 error type 3d
174 718 error type 5e
212 230 315 error type 1a
247b 970 error type 2a
252 647 error type 6e 663
260b 1237 error type 2a
309b 793 error type 2a
324 769 383 error type 7e
350b 1291 error type 3c
389 294 299 error type 1a
415b 535 error type 6e
459 710 error type 4e
471 181 660 error type 3c
536b 330 error type 8e
602 480 error type 4e
692 250 306 error type 1a
708b 424 error type 3c
711 505 error type 3c
747 590 error type 3a
782b 385 error type 3c
792 270 400 error type 1a
943 280 error type 4e 300
1031 736 657 error type 2a
Table count 26 15,406 13 5682 14
Total count 108 57,756 13 14,431 14
Total number of nucleotides sequenced 72,187
Total number of nucleotides cloned 1,044,638
Total percentage sequenced 6.9%

Error type 1 indicates last nucleotide deleted; error type 2, multiple nucleotides deleted; error type 3, nucleotide substitution; error type 4, single nucleotide deletion inside primer region; error type 5, single nucleotide deletion outside primer region; error type 6, multiple nucleotide deletions inside primer region; error type 7, nucleotide insertion outside primer region; and error type 8, nucleotide insertion inside primer region

a

Results in silent mutation

b

Entire gene sequenced

c

Results in mis-sense mutation

d

Results in non-sense mutation

e

Results in frameshift mutation