Table 1.
Mutation Sequence Data
TP ORF | Number of base calls from 5′ end of gene | Result | Number of base calls from 3′ end of gene | Result | |||||
---|---|---|---|---|---|---|---|---|---|
11 | 651 | 696 | error type 2a | ||||||
13b | 592 | error type 3c | |||||||
74 | 664 | error type 3c | 325 | error type 2a | |||||
117 | 874 | 678 | error type 3d | ||||||
174 | 718 | error type 5e | |||||||
212 | 230 | 315 | error type 1a | ||||||
247b | 970 | error type 2a | |||||||
252 | 647 | error type 6e | 663 | ||||||
260b | 1237 | error type 2a | |||||||
309b | 793 | error type 2a | |||||||
324 | 769 | 383 | error type 7e | ||||||
350b | 1291 | error type 3c | |||||||
389 | 294 | 299 | error type 1a | ||||||
415b | 535 | error type 6e | |||||||
459 | 710 | error type 4e | |||||||
471 | 181 | 660 | error type 3c | ||||||
536b | 330 | error type 8e | |||||||
602 | 480 | error type 4e | |||||||
692 | 250 | 306 | error type 1a | ||||||
708b | 424 | error type 3c | |||||||
711 | 505 | error type 3c | |||||||
747 | 590 | error type 3a | |||||||
782b | 385 | error type 3c | |||||||
792 | 270 | 400 | error type 1a | ||||||
943 | 280 | error type 4e | 300 | ||||||
1031 | 736 | 657 | error type 2a | ||||||
Table count | 26 | 15,406 | 13 | 5682 | 14 | ||||
Total count | 108 | 57,756 | 13 | 14,431 | 14 | ||||
Total number of nucleotides sequenced | 72,187 | ||||||||
Total number of nucleotides cloned | 1,044,638 | ||||||||
Total percentage sequenced | 6.9% |
Error type 1 indicates last nucleotide deleted; error type 2, multiple nucleotides deleted; error type 3, nucleotide substitution; error type 4, single nucleotide deletion inside primer region; error type 5, single nucleotide deletion outside primer region; error type 6, multiple nucleotide deletions inside primer region; error type 7, nucleotide insertion outside primer region; and error type 8, nucleotide insertion inside primer region
Results in silent mutation
Entire gene sequenced
Results in mis-sense mutation
Results in non-sense mutation
Results in frameshift mutation