Fig. 4.
Culture of B/I-activated lymphocytes in IL-7/15 increases proportion of CD44+CD62L+ double positive cells compared to IL-2. B16-GMCSF sensitized DLNs were harvested from pmel-1 mice and pulsed for 18 h at 1 × 106 DLN cells/ml in B (5 nM)/I (1 μM) + IL-2 (80 U/ml) or B/I + IL-7/15 (10 ng/ml each) then expanded in either IL-2 (40 U/ml) or IL-7/15 (10 ng/ml each), respectively. Samples were taken on days 3, 6, and 8 of expansion and incubated with mAb against CD44 and CD62L. Fluorescence of 15,000 cells per sample was analyzed by flow cytometry. Numbers in upper right quadrants represent percentage of double positive cells for day 8 samples for each culture condition in this experiment. Results are representative of three separate experiments